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Two major advances in the laboratory diagnosis are the use of GDH detection in stools as a means of screening for CDI and the development of NAATs such as PCR to detect toxigenic strains of C. difficile. Although GDH is sensitive, it is not as specific for CDI, because this enzyme is produced by both toxigenic and non-toxigenic organisms.

Additionally, antibodies against C. The sensitivity of GDH antigen detection has led to its use as a screening test as part of CDI testing algorithms, although it should be noted that as many as 10 of patients with toxigenic organisms can be missed by this method.